axis  (EXAKT Technologies)

Journal: Poultry Science

Article Title: Gut microbiota-derived taurolithocholic acid modulates myofiber-type switching via p38 MAPK/PGC-1α signaling underlying breed differences between Arbor Acres and Taoyuan chickens

doi: 10.1016/j.psj.2026.106914

Figure Lengend Snippet: Comparative analysis of growth performance and pectoral muscle development of postnatal broiler chickens (n = 6). A The sampling time of AA chickens at day 1 after hatching (D1), D7, D14, D21, D28, D42, D56 and TY chickens at D1, D7, D14, D21, D28, D42, D56, D77, D105. B The changes in body weight of AA and TY chickens. C The changes in pectoral muscle weight of AA and TY chickens. D Immunofluorescence staining of myofibers with MYH1A and MYH7B of AA and TY chickens. Scale bars: 20 μm. E Statistical analysis of immunofluorescence staining density values for pectoral muscle fibers in AA and TY chickens. Data were shown as mean ± SD; * P < 0.05, ⁎⁎ P < 0.01, ⁎⁎⁎ P < 0.001.

Article Snippet: Following blocking with 1% BSA, the sections were incubated with the primary antibody MYH1A (1:100, F59, DSHB, USA) and the AF647-labeled goat anti-mouse secondary antibody (1:500, A0473, Beyotime), with the primary antibody MYH7B (1:100, S58, DSHB) and the FITC-labeled goat anti-mouse secondary antibody (1:500, A0568, Beyotime).

Techniques: Sampling, Immunofluorescence, Staining

Journal: Poultry Science

Article Title: Gut microbiota-derived taurolithocholic acid modulates myofiber-type switching via p38 MAPK/PGC-1α signaling underlying breed differences between Arbor Acres and Taoyuan chickens

doi: 10.1016/j.psj.2026.106914

Figure Lengend Snippet: Intestinal microbiota transplantation (IMT) affects the growth and pectoral muscle development of chickens. A IMT experimental design. B The variation of body weight of AA and TY chickens during D1 to D28 (n = 6). C Pectoral muscle weight of AA and TY chickens at D21 and D28 (n = 12). d -E Morphology of the myofibers stained by hematoxylin and eosin and the statistics of muscle fiber diameter (MDia) and muscle fiber density (MDen) at D21( D ) and D28 ( E ). F-G The mRNA expression of MYH1A and MYH7B in the pectoral muscle at D21 ( F ) and D28 ( G ) (n = 6). Data were shown as mean ± SD; * P < 0.05, ⁎⁎ P < 0.01, ⁎⁎⁎ P < 0.001.

Article Snippet: Following blocking with 1% BSA, the sections were incubated with the primary antibody MYH1A (1:100, F59, DSHB, USA) and the AF647-labeled goat anti-mouse secondary antibody (1:500, A0473, Beyotime), with the primary antibody MYH7B (1:100, S58, DSHB) and the FITC-labeled goat anti-mouse secondary antibody (1:500, A0568, Beyotime).

Techniques: Transplantation Assay, Staining, Expressing

Journal: Poultry Science

Article Title: Gut microbiota-derived taurolithocholic acid modulates myofiber-type switching via p38 MAPK/PGC-1α signaling underlying breed differences between Arbor Acres and Taoyuan chickens

doi: 10.1016/j.psj.2026.106914

Figure Lengend Snippet: TLCA regulates mitochondrial biogenesis and altered myofiber type composition through the p38 MAPK/PGC-1α signaling pathway. A Cell viability of cells treated with different concentrations of TLCA for 24 h (n = 10). B & F The mRNA expression of MYH1A and MYH7B (n = 6). C & G Intracellular enzymatic activities of succinate dehydrogenase (SDH) and lactate dehydrogenase (LDH), ATP content and mitochondrial DNA (mtDNA) content (n = 6). D & H The mRNA expression of HK1, GPI, PGAM1, PGK1, PYGL, PGC-1α, NRF1 and TFAM (n = 6). E The protein expression of PGC-1α, p38 MAPK and phosphorylation p38 MAPK (n = 3). I The protein expression of PGC-1α (n = 3). Data were shown as mean ± SD; *Represents significant difference when compared with 0 μM TLCA or CON group, and # represents significant difference when compared with TLCA group. *& # P < 0.05, ⁎⁎ & ## P < 0.01, ⁎⁎⁎ & ### P < 0.001.

Article Snippet: Following blocking with 1% BSA, the sections were incubated with the primary antibody MYH1A (1:100, F59, DSHB, USA) and the AF647-labeled goat anti-mouse secondary antibody (1:500, A0473, Beyotime), with the primary antibody MYH7B (1:100, S58, DSHB) and the FITC-labeled goat anti-mouse secondary antibody (1:500, A0568, Beyotime).

Techniques: Expressing, Phospho-proteomics

Journal: Poultry Science

Article Title: Gut microbiota-derived taurolithocholic acid modulates myofiber-type switching via p38 MAPK/PGC-1α signaling underlying breed differences between Arbor Acres and Taoyuan chickens

doi: 10.1016/j.psj.2026.106914

Figure Lengend Snippet: Comparative analysis of growth performance and pectoral muscle development of postnatal broiler chickens (n = 6). A The sampling time of AA chickens at day 1 after hatching (D1), D7, D14, D21, D28, D42, D56 and TY chickens at D1, D7, D14, D21, D28, D42, D56, D77, D105. B The changes in body weight of AA and TY chickens. C The changes in pectoral muscle weight of AA and TY chickens. D Immunofluorescence staining of myofibers with MYH1A and MYH7B of AA and TY chickens. Scale bars: 20 μm. E Statistical analysis of immunofluorescence staining density values for pectoral muscle fibers in AA and TY chickens. Data were shown as mean ± SD; * P < 0.05, ⁎⁎ P < 0.01, ⁎⁎⁎ P < 0.001.

Article Snippet: Following blocking with 1% BSA, the sections were incubated with the primary antibody MYH1A (1:100, F59, DSHB, USA) and the AF647-labeled goat anti-mouse secondary antibody (1:500, A0473, Beyotime), with the primary antibody MYH7B (1:100, S58, DSHB) and the FITC-labeled goat anti-mouse secondary antibody (1:500, A0568, Beyotime).

Techniques: Sampling, Immunofluorescence, Staining

Journal: Poultry Science

Article Title: Gut microbiota-derived taurolithocholic acid modulates myofiber-type switching via p38 MAPK/PGC-1α signaling underlying breed differences between Arbor Acres and Taoyuan chickens

doi: 10.1016/j.psj.2026.106914

Figure Lengend Snippet: Intestinal microbiota transplantation (IMT) affects the growth and pectoral muscle development of chickens. A IMT experimental design. B The variation of body weight of AA and TY chickens during D1 to D28 (n = 6). C Pectoral muscle weight of AA and TY chickens at D21 and D28 (n = 12). d -E Morphology of the myofibers stained by hematoxylin and eosin and the statistics of muscle fiber diameter (MDia) and muscle fiber density (MDen) at D21( D ) and D28 ( E ). F-G The mRNA expression of MYH1A and MYH7B in the pectoral muscle at D21 ( F ) and D28 ( G ) (n = 6). Data were shown as mean ± SD; * P < 0.05, ⁎⁎ P < 0.01, ⁎⁎⁎ P < 0.001.

Article Snippet: Following blocking with 1% BSA, the sections were incubated with the primary antibody MYH1A (1:100, F59, DSHB, USA) and the AF647-labeled goat anti-mouse secondary antibody (1:500, A0473, Beyotime), with the primary antibody MYH7B (1:100, S58, DSHB) and the FITC-labeled goat anti-mouse secondary antibody (1:500, A0568, Beyotime).

Techniques: Transplantation Assay, Staining, Expressing

Journal: Poultry Science

Article Title: Gut microbiota-derived taurolithocholic acid modulates myofiber-type switching via p38 MAPK/PGC-1α signaling underlying breed differences between Arbor Acres and Taoyuan chickens

doi: 10.1016/j.psj.2026.106914

Figure Lengend Snippet: TLCA regulates mitochondrial biogenesis and altered myofiber type composition through the p38 MAPK/PGC-1α signaling pathway. A Cell viability of cells treated with different concentrations of TLCA for 24 h (n = 10). B & F The mRNA expression of MYH1A and MYH7B (n = 6). C & G Intracellular enzymatic activities of succinate dehydrogenase (SDH) and lactate dehydrogenase (LDH), ATP content and mitochondrial DNA (mtDNA) content (n = 6). D & H The mRNA expression of HK1, GPI, PGAM1, PGK1, PYGL, PGC-1α, NRF1 and TFAM (n = 6). E The protein expression of PGC-1α, p38 MAPK and phosphorylation p38 MAPK (n = 3). I The protein expression of PGC-1α (n = 3). Data were shown as mean ± SD; *Represents significant difference when compared with 0 μM TLCA or CON group, and # represents significant difference when compared with TLCA group. *& # P < 0.05, ⁎⁎ & ## P < 0.01, ⁎⁎⁎ & ### P < 0.001.

Article Snippet: Following blocking with 1% BSA, the sections were incubated with the primary antibody MYH1A (1:100, F59, DSHB, USA) and the AF647-labeled goat anti-mouse secondary antibody (1:500, A0473, Beyotime), with the primary antibody MYH7B (1:100, S58, DSHB) and the FITC-labeled goat anti-mouse secondary antibody (1:500, A0568, Beyotime).

Techniques: Expressing, Phospho-proteomics

Journal: medRxiv

Article Title: Single-Nucleus to Whole Body Phenotyping Reveals Neuromuscular Impairment and Preserved Exercise Adaptations in Long-Term Pediatric HSCT Survivors >10 years after treatment

doi: 10.64898/2026.04.24.26351644

Figure Lengend Snippet: (A) Representative magnetic resonance imaging (MRI) of the thigh and quadriceps muscle cross-sectional area (CSA) assessed by MRI (controls: n = 17; HSCT: n = 11). (B) Representative DXA scan and whole-body, and leg, lean mass assessed by DXA (controls: n = 27; HSCT: n = 17). (C) Whole-body bone mineral density and Z-scores assessed by DXA (controls: n = 27; HSCT: n = 17). (D) Isometric maximal voluntary contraction (MVC) measured by dynamometry, presented as Isometric MVC and Specific force (normalized to body weight) (controls: n = 28; HSCT: n = 18). Rate of force development (RFD) was also measured by dynamometry (controls: n = 11; HSCT: n = 7). (E) Physical function assessed using the 30-s chair-stand test, timed up-and-go test, and 6-min walk test (controls: n = 17; HSCT: n = 11). F-K. Data from muscle biopsy immunofluorescence, analyzed by two-way repeated-measures ANOVA with factors Group and Sex. Data are displayed as individual values with mean ± SD. Men are represented by circles, women by squares (controls: n = 26; HSCT: n = 16). (F) Muscle biopsy cross-section stained by immunofluorescence with the basement membrane marker laminin, delineating the muscle fiber borders, and type I myosin heavy chain (MyHC I). The unstained (black) fibers were designated type II fibers. Scale bar = 100 μm. Such images were used to calculate fiber type-specific data for G) fiber type distribution (proportion and relative area), H) shape factor index, I) fiber cross-sectional area (CSA), J) myonuclear content, K) myonuclear domain size. A-E. Data were analyzed with unpaired two-tailed t-tests.

Article Snippet: For assessment of denervated fibers, sections were stained for NCAM (AB5032; Sigma-Aldrich), dystrophin (D8168; Sigma-Aldrich), and MyHC I (A4.951; DSHB).

Techniques: Magnetic Resonance Imaging, Immunofluorescence, Staining, Membrane, Marker, Two Tailed Test